A chowdhary, s ahmad, zu khan, dc doval, hs randhawa. Gxm is also produced by species of the trichosporon genus, and both polysaccharides share antigenic determinants. Although the utility of azole therapy has been demonstrated in several observations, little is known about the efficacy of one of azoles, miconazole mcz. Isolation and antifungal susceptibility testing of. The patient was treated with amphotericin b and fluconazole, but t. This article is from brazilian journal of microbiology, volume 41. Trichosporon asahii, a non candida yeast that caused. Molecular targets and identification of trichosporon species in cultures 692. However, case reports of invasive trichosporonosis have been frequently published and the genus is. Most are typically isolated from soil, but several species occur as a natural part of the skin microbiota of humans and other animals. A fatal case of trichosporon asahii fungemia and pneumonia in. Trichosporon asahii sepsis in a patient with pediatric malignancy by aslinur ozkayaparlakay, eda karadagoncel, ali bulent cengiz, ates kara, atilla yigit, safak gucer and deniz gur get pdf 541 kb.
Infections by this species usually respond to treatment with azoles and amphotericin b. Leptosphaerulina chartarum unclassified fungi order capnodiales cladosporium herbarum rhodosporidium diobovatum trichosporon sp. Nov 1, 2017 fungi reads were from hortaea werneckii, aspergillus. Text is available under the creative commons attributionsharealike license. Gene ortholog relationships across trichosporon genomes were. Trichosporonosis definition of trichosporonosis by medical. Jul 19, 2019 asahii strain jcm 2466 was used instead of the sequence for strain cbs 2479. Trichosporon species, another yeast, have been reported as a cause of fungemia, especially in patients who have neutropenia and cancer. A fatal case of trichosporon asahii fungemia and pneumonia. Pulvirenti n, dalloglio f, greco am, oliveri s, schwartz ra, micali g.
The identity of all trichosporon species was confirmed by analysis of the intergenic spacer 1 region of the fungal ribosomal rna gene. Feb 12, 2018 all pathogenic members of the genus trichosporon were once regarded as a single species, t beigelii. Trichosporon asahii is the major causative fungus of disseminated or deepseated trichosporonosis and forms a biofilm on medical devices. Although most species are considered commensals of the human skin and gastrointestinal tract, these basidiomycetes are an increasing cause of fungal disease among immunocompromised hosts, such as hematological patients and solid organ transplant recipients. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a pdf plugin installed and enabled in your browser. Oct 17, 2016 trichosporon asahii was the main causative species 46. Trichosporon asahii infection in an advanced aids patient.
Trichosporon beigelii is a fungus once thought to cause only superficial infections, but recently has been increasingly identified as an opportunistic systemic pathogen in immunocompromised patients. Two case presentations infected by trichosporon asahii and. This system utilized 2 pan trichosporon probes designed to hybridize to the its2 region, which is highly variable among the genomes of individual fungal species, and it detected at least four clinically relevant trichosporon species t. This system utilized 2 pantrichosporon probes designed to hybridize to the its2 region, which is highly variable among the genomes of individual fungal species, and it detected at least four clinically relevant trichosporon species t. Disseminated infection due to trichosporon species is one of the emerging mycoses in neutropenic patients, particularly when they are treated for haematological malignancy. Although trichosporon is a causative pathogen of white piedra and summer. In this study, six strains, including two clinical isolates, from four different species trichosporon asahii, t. In 1902, vuillemin designated all trichosporon species trichosporon beigelii. Each strain produced a brownish pigment compatible with melanin. The patient was advised to keep the hair dry, and was treated with 2% ketoconazole shampoo topically daily for 2 months with oral fluconazole for 3 months.
Clinical breakpoints have not been defined for trichosporon species, and the mics cannot be reliably interpreted as indicating resistance or susceptibility. A fatal outbreak of trichosporon asahii sepsis in a neonatal intensive care unit. Structural and functional properties of the trichosporon. Trichosporon species isolated from the perigenital region. Full text a fatal case of trichosporon asahii fungemia.
Invasive trichosporonosis caused by trichosporon asahii and. The fungal genus trichosporon contains emerging opportunistic pathogens of humans, and is the third most commonly isolated noncandidal. Furthermore, four genotypes were identified in tunisia. For many years, the unique species of the genus, trichosporon beigelli, was only known as an environmental and saprophytic fungus occasionally found as the etiological agent of white piedra.
Trichosporon asahii, urinary tract infection trichosporon behrend is a genus of anamorphic yeasts yeast can be isolated from the sputum, the urine, the skin and basidiomycota, hymenomycetes, trichosporonales with the blood. In vitro antifungal susceptibility testing of the isolates showed that voriconazole exhibited the highest activity. Fungemia due to trichosporon asahii in a neutropenic child refractory to amphotericin b. By aslinur ozkayaparlakay, eda karadagoncel, ali bulent cengiz, ates kara, atilla yigit, safak gucer and deniz gur. A rare case of disseminated trichosporon asahii infection. The basidiomycetous yeast, trichosporon behrend, is a medically important genus that includes the causative agents of white piedra in immunocompetent hosts and disseminated infections in immunocompromised hosts. Trichosporon is a genus of anamorphic fungi in the family trichosporonaceae. Integrated transcriptomic analysis of trichosporon asahii. Invasive trichosporonosis is an extremely rare mycosis, but trichosporon fungemia tf in patients with hematologic malignancies has been increasingly recognized to be a fulminant and highly lethal infection.
Data from antifungal susceptibility testing ast studies were also. Infeccion urinaria nosocomial por trichosporon asahii. Frontiers the cell biology of the trichosporonhost. Trichosporon asahii was the most frequent species detected. Alternatively, you can download the file locally and open with any standalone pdf reader. There are now 100 reported cases of hematogenously disseminated infections with this lifethreatening yeast, and no effective antifungal therapy is available. Trichosporon asahii infection with rapid diagnosis mafiadoc. Differentiation of the emerging human pathogens trichosporon. Proliferation of trichosporon yeasts in the hair can lead to an unpleasant but nonserious condition. Biochemical and morphologic differences within the genus have led to the division of the former t beigelii into distinct species, at least 9 of which have the potential to cause human disease. Trichosporon asahii sepsis in a patient with pediatric malignancy. The plaque progressively increased in size, and similar plaques developed over the back, right dorsal surface of the hand, suprapubic area figure 1a, and right thigh over the last 4 years. Trichosporon asahii and trichosporon asteroides from plos.
Biofilm formation leads to antifungal drug resistance, so biofilmrelated infections are relatively difficult to treat and infected devices often require surgical removal. Trichosporon asahii is a rare cause of disseminated fungal infection in immunocompromised patients, presenting a diagnostic and therapeutic challenge. Superficial cutaneous trichosporon asahii infection in an immunocompetent host. Genomics and transcriptomics analyses of the oilaccumulating. Trichosporonosis definition of trichosporonosis by. Fungi of the genus trichosporon are increasingly recognized as causative agents of superficial and invasive fungal disease in humans. We noted a good evolution and disappearance of the casts after the treatment. To assess its therapeutic role, we retrospectively investigated 6 cases of tf in patients with acute leukemia receiving mcz containing regimens. First genotype identification of trichosporon asahii in. Utility of miconazole therapy for trichosporon fungemia in.
Primary cutaneous trichosporonosis responsive to voriconazole. May 06, 2014 a fatal case of trichosporon asahii fungemia and pneumonia in a kidney transplant recipient during caspofungin treatment meifang yang,1,2 hainv gao,1,2 lanjuan li1,2 1state key laboratory for diagnosis and treatment of infectious diseases, the first affiliated hospital, school of medicine, zhejiang university. There are only sporadic reports of infections caused by t. Trichosporon asahii infection with rapid diagnosis. The objectives of our study were species identification and genotyping of trichosporon isolates collected at the parasitology and mycology laboratory in sfax, tunisia. Trichosporon asahii sepsis in a patient with pediatric. Trichosporon spp trichosporon species are an uncommon but important cause of deep and disseminated infection.
Susceptibility tests were conducted by disk diffusion and broth microdilution. A 67 year old male presented with a widespread erythematous rash prior to sustaining a syncopal episode outside the dermatologistsof. Trichosporon asahii is an opportunistic fungus that causes infections in immunosuppressed patients. Nosocomial trichosporon asahii fungemia in a patient with secondary hemochromatosis. Genome and transcriptome evolve separately in recently. Trichosporon asahii was identified from blood cultures, confirmed by morphological examination on cornmeal agar and speciated by biochemical analysis api 20c. A woman in her 20s presented with a large erythematous indurated plaque involving the face and neck that had been first noted at age 2 years. Glucuronoxylomannan gxm is a well described virulence factor of pathogenic species in the cryptococcus. Full text a fatal case of trichosporon asahii fungemia and. In vitro antifungal susceptibilities of trichosporon species. Cmi gml n caso agente fecha cultivo recuento ufcml anfotericina b ketoconazol itraconazol fluconazol 1 t. We present two pediatric oncology cases successfully treated with voriconazole after t.
In our study, resistance to fluconazole was detected. Neutropenia developing due to malignancies is an important risk factor for fungal infection. Since only sporadic cases of breakthrough trichosporonosis have been reported. Superficial cutaneous trichosporon asahii infection in an immunocompetent host superficial cutaneous trichosporon asahii infection in an immunocompetent host pulvirenti, nella. Therefore, prevention of biofilm formation is important in clinical settings. Trichosporon asahii an overview sciencedirect topics. Melanization of members of the genus trichosporon is poorly described. Gene ortholog relationships across trichosporon genomes were determined using inparanoid version 4. First genotype identification of trichosporon asahii in sfax. Trichosporon asahii, has been reported increasingly in immunocompetent hosts. A fatal case of trichosporon asahii fungemia and pneumonia in a kidney transplant recipient during caspofungin treatment meifang yang,1,2 hainv gao,1,2 lanjuan li1,2 1state key laboratory for diagnosis and treatment of infectious diseases, the first affiliated hospital, school of medicine, zhejiang university. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a pdf plugin. Antifungal susceptibility profile of trichosporon isolates.
Molecular identification of trichosporon species pdf paperity. Abstractthe present study aimed to 1 determine the colonization rates of medically important. Methods for the diagnosis of invasive trichosporonosis include pcrbased methods, luminex xmap technology. Three contigs assembled from the trichosporon reads.
The aim of the present study was to evaluate the antifungal susceptibility profile of trichosporon species isolated from different sources employing the clinical and laboratory standards institute clsi method and etest method. A rare case report by shihta shang, yasung yang and mingyieh peng download pdf 183 kb. Scarring developed in the plaques over the face and hand during. Free fulltext pdf articles from hundreds of disciplines, all in one place differentiation of the emerging human pathogens trichosporon asahii and trichosporon asteroides from other pathogenic yeasts and moulds by using speciesspecific monoclonal antibodies pdf paperity. Molecular identification was carried out by analysing the igs1 regions of the rdna of 30 trichosporon isolates. Trichosporon inkin as an emergent pathogen in patients. Glucuronoxylomannan gxm is a well described virulence factor of pathogenic species in the cryptococcus genus. Trichosporon asahii formerly trichosporon beigelii is an emerging fungal pathogen seen particularly in immunologically compromised patients. Trichosporon asahii, a noncandida yeast that caused fatal. Despite the increased frequency and severity of trichosporonosis, data on the antifungal susceptibilities of trichosporon spp. By multivariate analysis, breakthrough infection or 2. Trichosporon asahii the most common cause of disseminated disease, trichosporon inkin the cause.
Trichosporon asahii candida albicans candida parasilopsis unclassified ascomycota family nectriaceae gibberella zeae hypocreales sp. Trichosporon mycotoxinivorans, a novel respiratory pathogen in patients with cystic fibrosis. Superficial cutaneous trichosporon asahii infection in an. Trichosporon asahii as an emerging etiologic agent of disseminated trichosporonosis. Trichosporon asahii among intensive care unit patients at a medical center in jamaica volume 34 issue 6 robyn neblett fanfair, orville heslop, kizee etienne, lois rainford, monika roy, lalitha gade, joyce peterson, heather oconnell, judith noblewang, s. Feb 12, 2018 a fatal outbreak of trichosporon asahii sepsis in a neonatal intensive care unit. Abstractthe fungal genus trichosporon contains emerging opportunistic pathogens of humans, and is the third most.
Trichosporon asteroides is an asexual basidiomycetous fungus first described from human skin but is now mainly isolated from blood and urine. Pdf trichosporon asahii formerly trichosporon beigelii is an emerging fungal pathogen. All species of trichosporon are yeasts with no known teleomorphs sexual states. Trichosporon asahii was the main causative species 46. Successful treatment of breakthrough disseminated trichosporon. The virulence attributes of trichosporon asahii are virtually unknown, despite its growing relevance as causative agent of superficial and invasive diseases in humans. A fatal case of trichosporon asahii fungemia and pneumonia in a kidney transplant recipient during caspofungin treatment meifang yang,1,2.
Molecular identification of trichosporon species pdf. An trichosporon asahii in nahilalakip ha genus nga trichosporon, ngan familia nga trichosporonaceae. Trichosporon inkin as an emergent pathogen in patients with. Antachopoulos c, papakonstantinou e, dotis j, bibashi e, tamiolaki m, koliouskas d.
An trichosporon asahii in uska species han fungi in nahilalakip ha divisio nga basidiomycota, ngan nga ginhulagway ni akagi, sugita, a. Trichosporon genus is subdivided into six distinct human pathogenic species of which t. A case report and an update a chowdhary, s ahmad, zu khan, dc doval, hs randhawa department of medical mycology, vallabhbhai patel chest institute, university of delhi, new delhi 110 007, india. However, case reports of invasive trichosporonosis have been frequently published and the. This was further confirmed by searches in the genomes of the t. Integrated transcriptomic analysis of trichosporon asahii uncovers the core genes and pathways of fluconazole resistance.
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